Whole proteome studied with 2D-LC-MS/MS

Liquid chromatography mass spectrometry processes have been used to investigate ABE fermentation between glucose and xylose substrates.

In a study published by Proteome Science and undertaken by scientists at McGill University in Quebec, a team sought to examine the shotgun proteomic profiling of C.acetobutylicum from ABE fermentation using glucose and xylose to understand the functional mechanisms of C.acetobutylicum proteins involved in butanol production.

The scientists identified 894 different proteins in C.acetobutylicum from ABE fermentation process using two dimensional - liquid chromatography - tandem mass spectrometry (2D-LC-MS/MS).

A total of 649 proteins were found to be common while 22 were be significantly differently expressed between glucose and xylose substrates.

"Our results demonstrate that flagellar proteins are highly up-regulated with glucose compared to xylose substrate during ABE fermentation," the report claimed.

"Chemotactic activity was also found to be lost with the xylose substrate," the team added, due to the absence of CheW and CheV proteins.