Method development for simultaneous analysis of Amyloid β (Aβ) proteins

Amyloid β (Aβ) are peptides of 36–43 amino acids that are the main components of amyloid plaques. The analysis of Aβ is not trivial as the Aβ peptides such as Aβ (1–42) are highly hydrophobic which leads to the formation of aggregates.

YMC has developed a method for the simultaneous determination of four Aβ using YMC-Triart Bio C4. As the hydrophobic Aβ have a tendency to form aggregates a stationary phase with lower hydrophobicity and a larger pore is needed. Therefore, YMC-Triart Bio C4 is the optimum column for this demanding separation. Due to the high chemical stability of YMC-Triart Bio C4 it is possible to do rapid method optimisation for complex mixtures of peptides and proteins utilising the wide pH and temperature range available. YMC-Triart Bio C4 is available with 3 and 5 μm for HPLC, as well as with fully scalable 1.9 μm for UHPLC.