Your Solution for the Purification of AAVs

Adeno-associated viruses (AAVs) are essential tools in gene therapy, but their size and complexity make purification challenging. MacroSep IEX Q by YMC is specifically designed to handle these challenges. Unlike conventional IEX resins, its macro-porous structure ensures efficient binding and separation of large biomolecules, including AAVs.

Why Choose MacroSep IEX Q for AAV Purification?

• Superior Separation Efficiency – Enables clear differentiation of full and empty AAV capsids.
• High Dynamic Binding Capacity (DBC) – Maximises loadability for improved process efficiency.
• High Flow Rate Performance – Maintains resolution even under demanding conditions.

Case Study: Separation of Full and Empty Capsids

Efficient and safe purification of biomolecules is critical but often presents significant complexity. One key step in the purification of AAVs is the separation of empty capsids from fully loaded capsids. These separations are essential to ensure therapeutic efficacy and safety.

Real-life data from an AAV2 purification process demonstrates that MacroSep IEX Q achieved excellent separation of full and empty capsids. Purity levels of the full AAV2 species were analysed before and after the chromatographic step using analytical ultracentrifugation. The AEX purification step with MacroSep IEX Q has increased the purity level of the target immensely. Starting from a purity of 54.4 % in the feed solution, the final purity after purification was 91.2 %.

Excellent Dynamic Binding Capacity for Large Biomolecules

Efficient purification processes with increased productivity rely heavily on high loadability. The DBC of a chromatography resin is a critical parameter. A higher DBC allows for greater loadability in packed columns, directly enhancing the overall process productivity. Large biomolecules, such as viral vectors, require resins with optimised, macro-porous structures for efficient binding. MacroSep IEX Q was specifically developed to meet these requirements, making it an effective solution for purifying large biomolecules.

High Productivity with Improved Pressure-Flow Characteristics

In addition to achieving high purity levels, the productivity of a purification process is strongly influenced by the pressure-flow properties of the resin. MacroSep IEX Q is based on hydrophilic polymer beads that exhibit exceptional pressure-flow characteristics, allowing its use at high flow rates without significantly compromising resolution. A direct comparison with a conventional IEX resin demonstrates that MacroSep IEX Q provides superior resolution, even under demanding conditions. Its macro-porous structure ensures reliable performance across both standard and elevated flow rates. This ability to maintain resolution at higher flow rates can substantially improve the throughput of DSP workflows.

Optimise Your DSP Process with MacroSep IEX Q

Need a high-performance resin for AAV purification? Contact us today to request a sample, discuss your application, or receive detailed technical data!