Chromatography used in nodulin study

Scientists have used quantitative analysis processes to characterise a novel late nodulin of the common bean with peptidase activity.

In a study published by BMC Plant Biology, scientists from institutions across Mexico noted that important roles in processes such as morphogenesis and metabolism have been assigned to nodulins during the legume-rhizobium symbiosis.

The legume-rhizobium symbiosis requires the formation of root nodules, which are specialised organs for the nitrogen fixation process, and nodule development is accompanied by the induction of certain specific plant genes, referred to as nodulin genes.

Using quantitative analysis processes, including chromatography, the team undertook the purification and biochemical characterisation of a novel nodulin from common bean (Phaseolus vulgaris L.) root nodules.

They determined that PvNod41 has limited peptidase activity against casein and is partially inhibited by pepstatin A.

The report added that the particular spatial and temporal expression patterns, as well as unique enzymatic properties, imply a high degree of functional specialisation.