• Column chromatography used in biochemistry research in India
    Biochemistry research in India used column chromatography to extract the active substance tuberosin

    Preparative

    Column chromatography used in biochemistry research in India

    Column chromatography has been used by scientists at Banaras Hindu University in India in biochemistry research into inflammation.

    Published in the Journal of Inflammation, the study looks at tuberosin from Pueraria tuberose in order to determine the nature of its known antioxidant activity.

    After using column chromatography to extract the active substance itself, the biochemistry researchers looked into a range of bioactivities associated with tuberosin.

    From their findings, they confirm that tuberosin can be considered as one of the active principal components in the antioxidant activity of Pueraria tuberose.

    Concentration-dependent inhibition of nitric oxide produced by lipopolysaccharides was also seen due to the inhibition of related protein expression.

    Metal chelation properties and scavenging potential seen in tuberosin for a variety of free radical compounds complete the significant conclusions of the study.

    The Journal of Inflammation affiliates itself with the British Inflammation Research Association and features submitted studies and commissioned non-research articles.

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