• The Basics of Mobile Phase Degassing

    HPLC, UHPLC

    The Basics of Mobile Phase Degassing

    The presence of air bubbles in the mobile phase of a liquid chromatography (LC) system can lead to a range of complications. For example, if the piston chamber becomes filled with air or the valve functions cannot be properly checked due to the presence of unwanted air, pumping reliability will be severely compromised. To prevent bubble formation, the mobile phase should be degassed. Generally an in-line degasser is the first choice, but sparging with helium can be an alternative if the mobile phase does not contain any volatile components. If air bubbles pass through the detector, then spurious peaks will be produced which will greatly affect the integrity of the results. For more information on why bubbles are a problem, check out the article: What is Mobile Phase Degassing? 

    Degassing Methods

    Luckily, degassing does not need to remove all of the excess dissolved air from a solvent for it to be effective. In fact, only enough has to be removed so that the level of gas in the mobile phase is less than the saturation level. This is normally around half of the gas present, which makes the process a relatively simple one. The four main methods of degassing are refluxing, helium sparging, vacuum degassing and sonication.

    Refluxing

    This is the most effective method of degassing, but unfortunately it is also the most difficult and the least practical as it involves boiling the solvent and distilling the gas. Because of its impracticality, this method is rarely used, although it yields near-perfect results.

    Helium Sparging

    This is a very effective and efficient method of degassing, which will remove more than 80% of dissolved gases and requires only one litre of helium to sparge the equivalent amount of mobile phase. However, prolonged sparging leads to distorted results, since some of the solution will inevitably evaporate in the process. As such, it is best only to use this method of degassing for a few minutes at a time.

    Vacuum Degassing

    Vacuum degassing can be achieved either by filtration prior to the LC process (the least effective method), via a batch-wise process treating the mobile phase itself (the most common method) or via on-line vacuuming, which utilises thin-walled porous tubing to permit vacuuming as part of the LC process itself (the most effective but most expensive method). In fact, as on-line vacuum degassing gains popularity, many LC system manufacturers are including vacuum degassing as a standard component of their LC system.

    As a general rule, vacuum degassing normally removes about 60% of unwanted dissolved gases, making it an effective method of degassing for most LC techniques.

    Sonication

    Ultra-sonic baths is not a viable standalone degassing method, since they can only remove about 30% of the dissolved gas present and at least 50% is required. However, when used in conjunction with either helium sparging or vacuum degassing, sonication is a useful method.


    Events

    Korea Lab 2025

    Apr 22 2025 Kintex, South Korea

    Analytica Anacon India & IndiaLabExpo

    Apr 23 2025 Mumbai, India

    AOCS Annual Meeting & Expo

    Apr 27 2025 Portland, OR, USA

    SETAC Europe

    May 11 2025 Vienna, Austria

    MSB 2025

    May 18 2025 Tempe. AZ, USA

    View all events

    Great Job...
    The latest issue will be with you shortly
    Sign up to Chromatography Today for FREE.
    Register and get the eBulletin, a Monthly email packed with the latest Chromatography products, news and services. Join us and get the latest information first.