New LC-MS method identified

Scientists have identified a non-targeted metabolic profiling system based on high resolution liquid chromatography-mass spectrometry (LC-MS) methods.

The team said that LC-MS needs to utilise the high-resolution power of an orbitrap, one of the most important analytical techniques for both metabolomics and proteomics, which boasts excellent mass accuracy.

It is therefore necessary to convert raw data to accurate and reliable m/z values for metabolic fingerprinting by high-resolution LC-MS.

In a study, published by BMC Bioinformatics, the scientists sought to create a novel, straightforward m/z detection method – which they called AMDORAP – to identify m/z values more accurately than other LC-MS analysis methods.

The team compared the m/z values of 14 identified compounds using AMDORAP and other LC-MS tools, finding that the new method provided fewer errors and greater levels of accuracy.

"AMDORAP allows us to address the relationships between biological effects and cellularmetabolites based on accurate m/z values," the scientists explained, adding that accurate m/z values are indispensable as a starting point for LC-orbitrap analysis.