LC-MS used in bladder cancer study

Scientists have used liquid chromatography-mass spectrometry to identify proteins in the study of bladder cancer cells.

The aim of the study, published in the Cancer Cell International journal, was to facilitate candidate biomarkers and improve therapy for muscle-invasive bladder transitional cell carcinoma.

Laser capture microdissection was used by the team to harvest normal urothelial cells and purified muscle-invasive bladder cancer cells from four paired samples and two-dimensional LC-MS was used to identify the proteome expression profile.

As a result of the investigation the team identified 42/45 GO terms as enriched and 9/5 as depleted, allowing the scientists to find significant differences between normal and cancer cells.

Furthermore, descriptive statistics show that PI Distribution of candidate biomarkers have certain regularity.

It has created a base for further study as it identified the proteome expression profile of muscle-invasive bladder cancer cells and normal urothelial cells which may aid the discovery of biomarkers and targeted therapy.