HPLC used in shock study

Scientists have used high performance liquid chromatography (HPLC) to discover the effect of fluid loading during hypovolemic shock on caspofungin pharmacokinetic parameters in pigs.

In a study published in Critical Care, the scientists behind the investigation explained that caspofungin treatment is frequently initiated in hypovolemic shock patients, with this study on pigs seeking to provide insight into the effects of fluid loading.

A dozen pigs were bled following anaesthesia and mechanical ventilation to introduce a two-hour deep shock and resuscitated using normal saline based on hemodynamic goals, while 16 animals were used as controls.

Using HPLC, the scientists discovered that extravascular lung water index was higher in the shock group, while maximal plasma concentration was 37 per cent lower than the control group, however plasma to tissue ratios were not different.

"Hypovolemic shock followed by fluid loading in pig results in a significant increase in the apparent volume of distribution of caspofungin and in a decrease in its plasma and pulmonary exposition," the report concluded.

It added that while the results were for capillary leakage and pulmonary edema, the same results would hold true for septic shock.