Preparative
SEC-Light Scattering for Biomolecular Characterisation
Dec 08 2011
Author: Ben MacCreath, Graham Cleaver and Greg Saunders on behalf of Agilent Technologies Europe
The area of biomolecular characterisation for molecular weight determination has increased rapidly with the requirements for a complete picture of the behaviour of biomolecules in solution being both critical not only for the academic understanding, but also from a regulatory viewpoint for safety and efficacy. For example with bioconjugates such as pegylated interferon, used in the treatment of chronic hepatitis, it is essential for the molar masses to be above that of the kidney cutoff to increase half-life and therefore efficacy.
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